基本信息
貨號(hào)：12500 (1mg/1mL)
儲(chǔ)存方式：保持在-20 oC。到期日為自收到之日起一年。
CAS號(hào)：61970-00-1
分子量：~60kDa
形式：液體（1mg / mL）
測(cè)量范圍：≤1飛摩爾ATP敏感度（使用0.2μg熒光素酶和適當(dāng)敏感的液體閃爍計(jì)數(shù)器或發(fā)光計(jì)）
生物應(yīng)用
該重組螢火蟲(chóng)熒光素酶是從來(lái)自北美螢火蟲(chóng)（Photinus pyralis）的克隆基因表達(dá)的酶，其提供使用生物發(fā)光試劑檢測(cè)ATP或熒光素底物進(jìn)行研究或生產(chǎn)試劑盒所需的可靠性和可靠性。 該重組酶可以潛在地消除可能在從天然來(lái)源純化的熒光素酶中發(fā)現(xiàn)的季節(jié)性和區(qū)域變異性的可能性。
該酶與熒光素，ATP和O2的反應(yīng)導(dǎo)致光的發(fā)射。 熒光素酶可用于檢測(cè)痕量的ATP。 螢火蟲(chóng)熒光素酶也是基因表達(dá)研究中*常用的報(bào)告基因之一。 熒光素酶催化的生物發(fā)光反應(yīng)是測(cè)量基因表達(dá)的*靈敏的分析工具之一。 通過(guò)使用0.2μg熒光素酶可以檢測(cè)小于或等于1飛摩爾的ATP。
參考文獻(xiàn)
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