Hoechst 34580細(xì)胞核染料，用于在熒光顯微鏡中標(biāo)記DNA。 因?yàn)檫@些熒光染色標(biāo)記DNA，它們也常用于可視化細(xì)胞核和線粒體。 通常使用這些密切相關(guān)的雙苯并酰亞胺中的兩種，Hoechst 33258和Hoechst 33342。 兩種染料都被約350nm的紫外光激發(fā)，并且都發(fā)射藍(lán)/青色熒光，發(fā)射值為461nm。 Hoechst染色劑可以與活細(xì)胞或固定細(xì)胞一起使用，并且通常用作另一種核酸染色劑DAPI的替代物。 它們之間的關(guān)鍵區(qū)別在于Hoechst 33342的額外乙基使其更具親脂性，因此更容易穿過完整的細(xì)胞膜。在某些應(yīng)用中，Hoechst 33258的滲透性明顯低于Hoechst 33342。這些染料也可用于通過繪制標(biāo)準(zhǔn)的發(fā)射 - 含量曲線來檢測(cè)樣品DNA的含量。百螢生物是AAT Bioquest的中國(guó)代理商，為您提供的Hoechst 33342細(xì)胞核染料。
Citations
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A circadian clock translational control mechanism targets specific mRNAs to cytoplasmic messenger ribonucleoprotein granules
Authors: Castillo, Kathrina D and Wu, Cheng and Ding, Zhaolan and Lopez-Garcia, Osiris K and Rowlinson, Emma and Sachs, Matthew S and Bell-Pedersen, Deborah
Journal: Cell Reports (2022): 111879
MDA5 Induces a Stronger Interferon Response than RIG-I to GCRV Infection through a Mechanism Involving the Phosphorylation and Dimerization of IRF3 and IRF7 in CIK Cells
Authors: Wan, Quanyuan and Yang, Chunrong and Rao, Youliang and Liao, Zhiwei and Su, Jianguo
Journal: Frontiers in Immunology (2017)
Grafting of Ring-Opened Cyclopropylamine thin films on Silicon (100) Hydride via UV Photoionization
Authors: Tung, Joline and Ching, Jing Yuan and Ng, Yoke Mooi and Tew, Lih Shin and Khung, Yit Lung
Journal: ACS Applied Materials & Interfaces (2017)
Dual-functionalized graphene oxide for enhanced siRNA delivery to breast cancer cells
Authors: Imani, Rana and Shao, Wei and Taherkhani, Samira and Emami, Shahriar Hojjati and Prakash, Satya and Faghihi, Shahab
Journal: Colloids and Surfaces B: Biointerfaces (2016): 315--325
A C-terminal acidic domain regulates degradation of the transcriptional coactivator Bob1
Authors: Lindner, John M and Wong, Christina SF and Möller, Andreas and Nielsen, Peter J
Journal: Molecular and cellular biology (2013): 4628--4640